Ch 20 PCR and seq - What we will cover: 1. What is the...

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What we will cover: 1. What is the polymerase chain reaction 1. What is gel electrophoresis 1. How is DNA sequenced
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1. What is the polymerase chain reaction
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How to create large quantities of DNA -process is Polymerase Chain Reaction (PCR) -used when small or impure DNA source -PCR quickly amplifies small amounts of DNA into large quantities -procedure can be completed in a few hours
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How is PCR accomplished? -3 step process -produces an exponentially growing population of DNA -each cycle DNA strands are heated to break hydrogen bonds -cooled to reanneal small primers -primers used to initiate DNA synthesis
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- primers are complementary to sequence that needs to be amplified -key to reaction is heat stable DNA polymerase -most enzymes do not stand temperatures of denaturization -heat stable DNA polymerase comes from prokaryotes that live in hot springs -entire life at denaturing conditions
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-normal DNA polymerase would be destroyed - heat stable polymerase
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This note was uploaded on 04/07/2008 for the course BIO Gen Bio taught by Professor Wallace during the Spring '08 term at Clarkson University .

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Ch 20 PCR and seq - What we will cover: 1. What is the...

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