BL Write Up 7 Fermenation and Respiration

BL Write Up 7 Fermenation and Respiration - Thuy Nguyen Bio...

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Thuy Nguyen Bio Lab 5A- 009 TA- Kai-Ti Lin 02.26.08 Lab 7 Fermentation and Respiration Write Up Q UESTION 1 How will increasing the number of yeast cells affect the rate of ATP production by the cells (in moles ATP/ hr) if the sugar concentration in the experiment is held constant? Hypothesis If the sugar concentration is held constant, increasing the number of yeast cell would increase the rate of ATP production by the cells as long as there is enough sugar. Supporting Facts - Fermentation is a degradation of sugars that occurs without oxygen (Campbell. “Biology”. 7 th ed. Text pp. 161). - Fermentation is an anaerobic alternative for producing ATP by harvesting the chemical energy of food (Campbell. “Biology”. 7th ed. 175). - Cell uses the energy released from the exergonic electron fall to regenerate its supply of ATP (Campbell. “Biology”. 7 th ed. Text pp. 164). - Yeast ferment sugars to alcohol and CO 2 under anaerobic conditions (Campbell. “Biology”. 7 th ed. Text pp. 623). - Yeast can make ATP to survive using fermentation or respiration (Campbell. “Biology”. 7 th ed. Text pp. 176). 1
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- Yeast also carries out alcohol fermentation, which regenerates the supply of NAD + needed for the continuation of glycolysis (Campbell. “Biology”. 7 th ed. Text pp. 175). - As long as there is a sufficient supply of NAD + to accept electrons, fermentation can generate ATP solely by substrate-level phosphorylation (Campbell. “Biology”. 7 th ed. Text pp. 174). Citation Campbell, Reece. “Biology”. 7 th ed. Textbook. San Francisco. Pearson. Oross, John. Fermentation and Respiration. Bio5LA Lab Manual 7. Winter '08 ed. UCR. Experimental Strategy We will use the manometer to measure the volume of gas consumed or produced by metabolizing cells, tissues. We will use three fermentation samples with three different sugar concentration and amount of yeast. The manometer will detect volume changes in the system that result from temperature-induced gas expansion or contraction. It will give us the volume
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This note was uploaded on 04/07/2008 for the course BIO Bio 5A taught by Professor Zhu,rao during the Winter '08 term at UC Riverside.

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BL Write Up 7 Fermenation and Respiration - Thuy Nguyen Bio...

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