Lecture Assignment 2

Lecture Assignment 2 - H. None of these I. Has 5’ to 3’...

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Biology 202 Lecture Homework 2 Due in Discussion Class - Week 3 (Jan. 30-Feb. 3) 1. DNA repair enzymes preferentially repair mismatched bases on the newly synthesized DNA strand, using the old DNA strand as a template. If mismatches were simply repaired without regard for which strand served as template, would this reduce replication errors? 2. The following events, steps, or reactions occur during E. coli replication. For each entry in Column A, select the appropriate entry in Column B. Each entry in Column A may have more than one answer, and each entry in Column B may be used more than once. Column A Column B A. Unwinds the double helix A. Polymerase I B. Prevents reassociation of complementary bases B. Polymerase III C. Is an RNA polymerase C. Helicase D. Is a DNA polymerase D. Primase E. Replaces RNA primers E. Ligase F. Is the major elongation enzyme F. SSB protein G. Is a 5’ to 3’ polymerase G. Topoisomerase H. Is a 3’ to 5’ polymerase
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Unformatted text preview: H. None of these I. Has 5’ to 3’ exonuclease activity J. Has 3’ to 5’ exonuclease activity K. Bonds free 3’OH end of a polynucleotide to a 5’ end of another polynucleotide L. Bonds 3’OH end of a polynucleotide to a free 5’ nucleotide triphosphate M. Relieves tension in unwinding DNA 3. DNA polymerases in all organisms only add 5’ nucleotides to the 3’ end of a growing DNA strand, never to the 5’ end. One reason for this is the fact that most DNA polymerases have a proof-reading function. a. Draw out the reaction that DNA polymerase would have to catalyze if DNA synthesis occurred in the 3’ to 5’ direction. Your drawing does not need to be detailed but be sure to include the location of hydroxyl groups and phosphates (mono- or tri-) in the drawing. b. Why would proofreading be problematic if DNA synthesis occurred in the 3’ to 5’ direction?...
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This homework help was uploaded on 04/07/2008 for the course BIOL 202 taught by Professor Staff during the Fall '08 term at New Mexico.

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Lecture Assignment 2 - H. None of these I. Has 5’ to 3’...

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