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Unformatted text preview: H. None of these I. Has 5’ to 3’ exonuclease activity J. Has 3’ to 5’ exonuclease activity K. Bonds free 3’OH end of a polynucleotide to a 5’ end of another polynucleotide L. Bonds 3’OH end of a polynucleotide to a free 5’ nucleotide triphosphate M. Relieves tension in unwinding DNA 3. DNA polymerases in all organisms only add 5’ nucleotides to the 3’ end of a growing DNA strand, never to the 5’ end. One reason for this is the fact that most DNA polymerases have a proof-reading function. a. Draw out the reaction that DNA polymerase would have to catalyze if DNA synthesis occurred in the 3’ to 5’ direction. Your drawing does not need to be detailed but be sure to include the location of hydroxyl groups and phosphates (mono- or tri-) in the drawing. b. Why would proofreading be problematic if DNA synthesis occurred in the 3’ to 5’ direction?...
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