molecular biology

Molecular biology - Molecular Biology Lab BIOSC 0060 Description of Data Escherichia coli(E coli were planted on LB(Luria Broth agar plates as a

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Molecular Biology Lab BIOSC 0060 Description of Data Escherichia coli (E. coli) were planted on LB (Luria Broth) agar plates as a way to discover which plasmid they were transformed by. Table 1 contains the expected results when E. coli is transformed by certain plasmids and plated on the LB agar plates. According to the data in the table, E. coli transformed by pAMP will grow on the LB, LB-AMP, and LB-AMP/ARA plates. E. coli transformed by pKAN will grow on the LB and LB-KAN plates. E. coli transformed by pGLO will grow on the LB, LB-AMP, and LB-AMP/ARA plates and will glow on the LB-AMP/ARA plate. The unknown grew on the LB, LB-AMP, and LB-AMP/ARA plates. In Table 2, the fragment lengths of Lambda DNA are presented along with their relative mobility. The fragment lengths of the unknown are also found by lining up the values for relative mobility against the trend line. It is clear by observing the data that as the fragment lengths increase the relative mobility decreases in the process. Table 3 contains standard and expected values of fragment lengths for various plasmids. The expected fragment lengths for pAMP were 3755bp and 784bp. The expected fragment lengths for pKAN were 2332bp and 1875bp. The expected fragment lengths for pGLO were 4496bp, 399bp, 226bp, 220bp, 30bp. The values for the unknown were measured to be 3500bp and 575bp. The image of the gel in Figure 1 is that of the pAMP.
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This note was uploaded on 04/07/2008 for the course BIOSC 0060 taught by Professor Barbarahertel during the Spring '07 term at Pittsburgh.

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Molecular biology - Molecular Biology Lab BIOSC 0060 Description of Data Escherichia coli(E coli were planted on LB(Luria Broth agar plates as a

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