Lecture 1 - enzyme kinetics

Also labeled is methionine 222 which is t he residue

Info iconThis preview shows page 1. Sign up to view the full content.

View Full Document Right Arrow Icon
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: ases. Also labeled is methionine 222 which is t he residue identified by peptide mapping studies t o be oxidized t o the sulfoxide by H P02 with res ultant inactivation (Stauffer nd Etson, a 1969). S ubstrate binds from t he N t o C terminus across t he binding cleft extending from t he upper right t o lower left (Robertus et al., 1972). TABLE I \ { mef -222 kc/ v r ground secreted protease activity Relative specificactivities of codon 222 m utant subtilisins creted proteases. Furthermore, Estell et al., 1985. Engineering an Enzyme by Site-directed Mutagenesis to Be Resistant to Chemical Oxidation. M utant enzymes were purified J.aBiol. assayed a6518-6521. nd Chem. 260: s described under tween t he plasmid containing the “Materials a nd Methods.” the host subtilisin gene can be av Relative specific To determine t he specific activ Codon 222 activity enzymes were purified from cult Enzyme Kinetics! Case Study - Genetic Engineering of Subtilisin! Subtilisin added to laundry detergents to clean protein food stains from fabri...
View Full Document

Ask a homework question - tutors are online