Unformatted text preview: if S and P have different light absorbing properties Enzyme Kinetics!
Case Study - Genetic Engineering of
2 NO Example: Subtilisin Assay (in case study handout)
Substrate: synthetic, artificial (synthesized in lab) & colourless + =O =O O
O- O N – Succinyl – Ala – Ala – Pro – Phe – p - nitroanaline 2 O - P O H2N - O NO2 OH 2
NO NO Product is Yellow O OH
- NO2 Subtilisin H + 2O Peptide linkage
hydrolyzed by subtilisin
2 H C – (CH2)2 – C – N – Ala – Ala – Pro – Phe – C –NN2 O
H + + Product liberated p-nitroanaline HP -2
O4 4 HP
O Enzyme Kinetics!
Effects of [substrate] on Reaction Rates! What will a substrate progress curve look like for each test tube?
W hich will have the largest reaction rate?
Substrate 2 3 Enzyme Kinetics!
Effects of [substrate] on Reaction Rates!
100% [S] Vo % of
Rate Time (s) Recall: rate = [S]o (mM) ∆[S]
∆t = rise
run units (mM)
(s) Enzyme Kinetics!
Effects of [Substrate] on Reaction Rates!
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This note was uploaded on 08/13/2013 for the course BIOC 302 taught by Professor Maurus during the Summer '09 term at UBC.
- Summer '09