{[ promptMessage ]}

Bookmark it

{[ promptMessage ]}

Lecture 1 - enzyme kinetics

Problem subtilisin is inactivated by bleach peroxide

Info iconThis preview shows page 1. Sign up to view the full content.

View Full Document Right Arrow Icon
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: cs. Problem: Subtilisin is inactivated by bleach (peroxide) in laundry detergent. This is due to oxidation of active site residue Met-222 & the formation of methionine sulfoxide. Oxidized Met interferes with important reactive residues. Enzyme Kinetics! Case Study - Genetic Engineering of Subtilisin! Site-Directed Mutagenesis. Solution: Use genetic engineering (site-directed mutagenesis) to replace Met-222 with another residue that does not react with peroxide yet P enzyme of function. still allows the roductionto a n Oxidatively Resistant Subtilisin F IG. 1. Stereoscopic view of t he active site of subtilisin from B . a m- \ kc/ yloliquefaciena s tructure (Wright et a l.. 1969). Residues labeled include NEED to put numbers to 32 serine 221, histidine 64, a nd aspartate which form t he function to enzyme catalytic triad typical of serine proteases. Also labeled is methio222 which is t he residue identified nineunderstand effects by peptide mapping studies t o be oxid f changing amino acyloized to the sulfoxide by HP02 with res ultant inactivation (Stauffer nd Etson, a 1969)...
View Full Document

{[ snackBarMessage ]}

Ask a homework question - tutors are online