Lecture 1 - enzyme kinetics

The vo versus s curve is a rectangular hyperbola

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Unformatted text preview: described by: y=a x +x Vmax = dependent on enzyme concentration vo = Vmax x b+x a vo = Vmax y [S] Km = concentration at half of Vmax Km + [S] [S] Km + [S] x b In the context of enzymology, the equation is called the Michaelis-Menten (M-M) equation. Enzyme Kinetics! Case Study - Genetic Engineering of Subtilisin! Table II: Kinetic Constants for selected Codon 222 mutants Codon 222 Kcat (S-1) Km (M) Kcat / Km (M-1 S-1) Met (wild-type) Cys Ser Leu 50 84 27 5 1.4 x 10-4 4.8 x 10-4 6.3 x 10-4 2.6 x 10-4 36x104 20x104 4x104 2x104 Enzyme Kinetics! Getting Numbers From Curves! 1 K 1 1 vo = m Vmax [S] + Graphical Estimation of Vmax (Kcat) and Km y= m Vmax Vmax x+b Double reciprocal plot Lineweaver-Burk plot -1 Km *lineralizing data adds some error Intercept on y-axis Intercept on x-axis 1 concentrations near 0 influence the slope of graph more than the concentrations near Vmax You ll gain greater insight in tutorial. Enzyme Kinetics! Case Study - Genetic Engineering of Subtilisin! Table II: Kinetic C...
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This note was uploaded on 08/13/2013 for the course BIOC 302 taught by Professor Maurus during the Summer '09 term at UBC.

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