Saturating conditions we cannot measure maximum

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Unformatted text preview: ng conditions We cannot measure maximum reaction rate directly. But, a V vs S curve approaches Vmax under saturating conditions.. Vmax Vo Initial Rate Enzyme Substrate 10 50 [S]o (mM) 100 Enzyme Kinetics! Effects of [Substrate] on Reaction Rates! Vmax Vo Enzyme Substrate Initial Rate 10 50 [S]o (mM) 100 Summary 1.  Reaction rate is faster if you have more initial substrate 2.  You approach maximum reaction rate for an enzyme-catalyzed reaction by trying to saturate E with S Enzyme Kinetics! Effects of [Enzyme] on Reaction Rates! saturating conditions How does [E] affect reaction rates? Vmax Vo Initial Rate Enzyme Substrate 10 50 [S]o (mM) 100 Enzyme Kinetics! Effects of [Enzyme] on Reaction Rates! [E] = 8 M Vo [E] = 4 M Vo Vo [S] [E] = 2 M [S] [S] The amount of enzyme ([E]) affects the maximum reaction rate. Enzyme Kinetics! Effects of [Enzyme] on Reaction Rates! Vmax: The rate when all of active sites of all enzyme molecules in the assay solution are always filled with substrate over the time the reaction rate is measured. The amount of enzyme ([E]) affects t...
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This note was uploaded on 08/13/2013 for the course BIOC 302 taught by Professor Maurus during the Summer '09 term at The University of British Columbia.

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