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8) Wear shoes at all times in the lab (shoes with closed toes are required for the lab on Genetic Transformation).
9) Cover any cuts or scrapes with a sterile, waterproof bandage before attending lab.
10) Do not perform unauthorized experiments.
11) Report all spills and accidents (including animal bites) to your instructor immediately.
12) Never pipette by mouth.
13) Wash skin immediately and thoroughly if contaminated by chemicals or microorganisms and notify your lab
14) Safety with hot plates or open flames:
a) Always assume that hot plates are hot.
b) Never leave heat sources unattended.
c) Be careful to avoid letting hot H20 baths boil dry.
d) Use appropriate apparatus when handling hot glassware.
e) Keep chemicals away from direct heat.
f) Keep flammable liquids (alcohol, acetone, etc.) away from open flames.
15) Do not allow any liquid to come in contact with electrical cords. Handle electrical connectors with dry hands. Do
not attempt to disconnect electrical equipment that crackles, snaps or smokes.
16) Always place used or waste materials in the area or container designated by your TA.
17) Do not pick up broken glassware with your hands. Also never dispose of broken glass or other sharp objects in the
18) Always wear disposable gloves when advised to do so by your TA.
19) Leave the laboratory clean and organized for the next student.
20) Students will not handle compressed gasses/regulators.
21) The departmental Chemical Hygiene Plan (CHP) and the Injury and Illness Prevention Program (IIPP) manual are
available in the labs and may be consulted for further safety information. xii Biology 05LA – Fall Quarter 2012 Lab 1 – page 1 LAB 1: INTRODUCTION TO LIGHT MICROSCOPY: THE COMPOUND
MICROSCOPE: ITS CARE, COMPONENTS AND PRINCIPLES OF USE.
Over the past 20 or so, the light microscope has become an extremely valuable tool in modern
cell and molecular biology. In fact, a substantial part of the lecture content of Biology 5A that you will
be hearing this quarter is derived from studies utilizing various forms of light microscopy. A feeling
for the importance of light microscopy can be gained with a brief scan of your text book (Biology,
Campbell et. al., 9th ed.). On page 113, the intracellular distribution of three classes of cytoskeletal
elements is shown by the use of fluorescent tags. On page 110, the distribution of DNA within a
mitochondrion is revealed by another fluorescence technique. The localization of telomeres on a
chromosome is shown on page 319. And finally, the dynamics of the cytoskeleton as it participates in
the process of mitosis is vividly demonstrated on pages 232 and 233. These few examples should
convince you of the power of modern light microscopy. While you will not be using any of these
techniques in Biol 05LA, it is very likely that you may later in your undergraduate studies or
thereafter. It is the purpose of this laboratory to prepare you for this eventuality.
The focus of this exercise will be in two areas. The first will be in the area of basic
microscopy. Here you will be introduced to the parts of the microscope and how these parts interact to
form an image. Then you will be introduced to some basic principles of light microscopy that are
essential in all forms of microscopy. We will conclude by providing you with some basic guidelines
for microscope use that have relevance to all
forms of light microscopy. The second area
of focus for this lab will be about how to
handle the microscope in a manner that will
not damage it. Microscopes are expensive
and obtaining the best possible results in
microscope studies requires that the
instrument being used is in top working
order. Please take heed of the safety guide
(1 of 4)
lines that will be presented as you work
through this exercise – your success in
Biology 05LA and future studies will be
enhanced if you do so.
I. MICROSCOPE COMPONENTS For Biol 05LA, the microscopes you
will use will be placed on the lab benches by
the lab prep. staff. Thus you will not need to
move them at the start or the end of the lab
period. Fine focus
Stage motion controls
Brightness control Illuminator Base The first thing you will need to do is
to look at Fig. 1 and familiarize yourself
with the names of the parts of the
microscope; return to this diagram as often
Figure 1. Parts of the compound light microscope;
as necessary so that you can learn these
The Nikon Alphaphot.
names. If the microscope at your bench is
not a Nikon Alphaphot, a labeled diagram appropriate for your microscope will be provided. Biology 05LA – Fall Quarter 2012 Lab 1 – page 2 Structural elements.
1. Binocular eyepiece tube. This assembly holds the ocular lenses. It is designed to allow
adjustment of the distance between the oculars and the focus of one of them. The eyepiece tube is
held in position by a setscrew (no...
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- Fall '12