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Liquid-Liquid Fraction of a Mixture

Liquid-Liquid Fraction of a Mixture - 1 Graham Barnett...

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Graham Barnett, Jillian Daviau, Nick Baccari PHRM235 Sec.2 Liquid-Liquid Fractions of a Mixture Aim: The purpose of this experiment was to use the different properties of a lipophilic, an acidic, a phenol, a basic, and a polar neutral compound to separate them into different fractions. Data: Table 1: TLC plate #1 Fraction Observed distance traveled in cm [a] Solvent front (cm) [d] Rf = a/d 1 7.10 7.50 0.95 2 2.30 7.50 0.31 3 3.20 7.50 0.41 5 2.25 7.50 0.30 Table 2: TLC plate #2 Fraction Observed distance traveled in cm [a] Solvent front (cm) [d] Rf = a/d 1 7.10 7.30 0.97 2 2.70 7.30 0.37 3 3.30 7.30 0.45 5 2.25 7.30 0.31 Tes-tape results from Fraction #4 Results: positive Original indicator’s color: blue Tested color: Light blue Concentration of Glucose for color indicated: Between 0 & 100 mg/dL Table 3: Fraction composition results Fraction Component Average RF value Chemical Properties 1 Sudan III 0.96 Lipophilic 2 Resorcinol 0.34 Phenol 3 Benzoic acid 0.43 Acid 4 Glucose N/A Polar Neutral 5 Brucine 0.305 Base Discussion: Through conducting this experiment the chemical composition of each fraction was determined by using the known lipophilic properties of Sudan III, Resorcinol, Benzoic acid, Glucose and Brucine. 1
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When first mixed with ethyl acetate (a hydrophobic solution) and HCl (an acidic and polar solution) the initial mixture containing the 5 compounds was able to become separated into 2 distinctive layers. The lower layer known as the aqueous layer contained the more dense hydrophilic solutions while the top ethyl acetate layer contained the less dense lipophilic solutions. Due to the nature of lipophilic and hydrophilic solutions wanting to keep the total entropy of the solution as small as possible the lipophilic solutions separate out from the hydrophilic solutions. The aqueous solution contained fractions 4 & 5. The ethyl acetate layer contained solutions 1, 2 & 3. At this point the two layers could be separated from one another.
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