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Unformatted text preview: • • Fermentation involves glucose oxidation to pyruvate and the donation of hydrogens (H+ + e­) from NADH + H+ to pyruvate or a derivative of pyruvate. Only one oxidation producing NADH and two SLP to make ATP Uses two ATP to start pathway,produce two more at two SLP steps above. Only 2 net ATP produced Rate is fast because so little energy is produced No oxygen, therefore no Krebb’s (TCA) cycle No ATP from OP. NADH is used to re­reduce pyruvate or a derivative Embden­Meyerhof pathway (EM), also known as gycolysis is a good example Bacterial Respiration Bacterial Respiration o Aerobic Respiration uses oxygen as a terminal (external) electron acceptors o o o o Pyruvate (3C) is oxidized to acetyl­CoA (2C) which enters the TCA (Krebb’s) cycle by condensing with the 4 C oxaloacetate to produce citric acid 4 moles of NADH & 1 mole of FADH2 are produced per pyruvate Since there are two pyruvates per starting glucose, this produces 30 net ATP When the two SLP ATP are added from the EM pathway and the NADH goes to electron transport to produce 3 X 2 = 6 more. These add to the 30 to give 38 total ATP per prokaryotic cell ATPase:ATP Synthase ATPase:ATP Synthase Catalyst for conversion of proton motive force into ATP F0 proton conducting channel ab2c12: Protons channel across membrane between a and c12 subunits. Proton movement drives rotation of c proteins, generating a torque transmitted to F1 by the gamma/ep subunits causing conformational change in beta subunits. F1 mutisubunit headpiece located on cytoplasmic side of membrane that catalyzes synthesis of ATP: Alpha3beta3gamma ep delta Three Types of Biological Oxidation Three Types of Biological Oxidation (Chemoorganotrophs) Types of Biological Oxidation for Chemoorganotrophs I. Fermentation Organic pyruvate/derivative e­ II. Aerobic Respiration III. Anaerobic Respiration Anaerobic Respiration Anaerobic Respiration • Anaerobic Respiration • • • • • Biological oxidation in which the terminal electron acceptor is usually an inorganic molecule (external) other than oxygen. Uses oxidative phosphorylation for ATP synthesis Some of the electron acceptors used include nitrate, sulfate and carbon dioxide. When carbon dioxide is used as an electron acceptor, the resulting product is either methane or acetic acid, depending on the organism involved. Much if not all the methane produced in our gut or by cows or in swamps is produced by this process. So when you eat too many beans.....blame the anaerobic bacteria Glycolysis: conversion of glucose to pyruvic acid. Both fermentation and aerobic respiration convert glucose to pyruvic acid, but two processes differ from there. FERMENTATION AEROBIC RESPIRATION 1. Fate of Pyruvic Acid Pyruvate or a derivative of pyruvate accepts the HÕsrom NADH + H+ f Conversion to AcetlyCoASH and entry into TCA cycle 2. Fate of NADH + H+ from glycolysis Donates HÕ to pyruvic acid s Donates HÕ to electron s transfer chain: produces 3 X 2 A TP 3. Terminal e- acceptor Pyruvate or derivative of pyruvate (internal intermediate of pathway Oxygen (O2) 4. Mechanism of ATP synthesis Substrate level phosphorylation (SLP). No oxidative phosphorylation. SLP and...
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This document was uploaded on 09/17/2013.

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