Lectures 25 Oxidative Phosphorylation-BW

In losse c onformation t coformation converts adp and

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Unformatted text preview: mation converts ADP and phosphate into ATP. ATP is tightly bound not going to come off by itself. need movment of protons - in open form ATP relased. block proton movemnt in T c onformaint 4/12/13 would not get ATP release. not only to drive 2000 MCB c ofnormational change but to release ATP. Lecture 25 Binding Proton enters cytoplasmic halfchannel; COO- is protonated; c ring rotates and proton exits matrix halfchannel and COO- restored. Proonation on aspartic acid - two half channels one where it enteres and one where it exits. as one becomes protonated rotates until lines up with the second half channel - this allows for the deproratation and entry. COObecomes protonated 4/12/13 4/12/13 Formed two moleucles of NADH. add up NADH - 2 from glycolysis 2 from pruvate dehydrogenase complex 6 from TCA ccyel and 2 molecules of FADH2. FADH2 mult. by 1.5 and NADH by 2.5. should get around 30. NADH doesn't cross mithcondria membrane dont get the same number from glycolysis - NADH needs to MCB 2000 Lecture 25 transfer though elctrons to some other molecule that can prop inner mithcondria membrane - results: difference because of the shuttle. NADH needs to be able to use a shuttle to transfer electrons. and bring into mitocdonria. Regulation of Oxidative Phosphorylation Electron transfer and ATP synthesis are tightly coupled Blocking the electron transport chain at any point prevents ATP synthesis. Dissipation of the proton gradient leads to uncoupling of oxidative phosphorylation Rate of ATP synthesis is co-ordinated with the rate of ATP utilization the rate of oxygen consumption. ATP synthesis and elctron trasnport are coupled - inhbit one for the other get no ATP syntehsis - inhibit ATP synthase - will block elctron trasnport.what happens if you destory the proton gradient? - inner membrane damaged in anyway - cant mantain proton gradient - cant synthesis ATP. electron trasnport chain will keep going. can uncoupled ATPsyntahse from eelctron trasnport. in additon: focus on ATP syntehsis dependent on rapid its used. muslce actively contacty - need more ATP and ATP can be slowed down. oxygen cnsumption and ATP synthesise are linked. 4/12/13 MCB 2000 Lecture 25 fi nal electron acceptor - oxygen consumption - linked to rate of ATP synthesis. Respiratory control: ADP concentration controls rate of O2 consumption elctron trasnport chain can detect c hanges in membrane potenial c ontrols how fast they move through ATP syntehis NAD+ returns to TCA cycle ATP synthase dissipates H+ gradient ETC generates H+ gradient ADP controls rate of oxygen consumption .high ADP - low energy charge and need to syntehsis ATP. ADP is your sensory molecule - indiciative of low charge. ADP high then ATP low. mithcondira need to s yntehsis ATP. plateu off. ATP is being consumed for muscle contration- then have high ADP. speed up the TCA cycle - consume more oxygen as the elctrons pass. oxygen consumtion rate increases. Electrons do not flow from fuel oxidation to O2 unless ATP needs to...
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This note was uploaded on 09/17/2013 for the course MCB 2610 / 200 taught by Professor Feldman during the Fall '12 term at UConn.

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