REVIEW pre Culture conditions for hESC

Organizations which fund hesc research it is an

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Unformatted text preview: mpt to study the properties of several hESC lines in similar conditions. A large range of hESC lines will be evaluated using the same antibodies and same laboratory systems in selected laboratories. However, so far it has not been possible to choose the most optimal conditions for these comparisons and further optimization and standardization of culture methods is needed for research as well as for clinical purposes. Acknowledgements Our units are supported by grants from the Academy of Finland, TEKES the Finnish Funding Agency for Technology and Innovation, The Competitive research funding of the Pirkanmaa Hospital District and the Swedish Research Council. The authors declare that there is no conflict of interest that would prejudice the impartiality of this scientific work. Reproduction (2006) 132 691–698 References Allegrucci C & Young LE 2006 Differences between human embryonic stem cell lines. Human Reproduction Update [in press]. Amit M, Carpenter MK, Inokuma MS, Chiu CP, Harris CP, Waknitz MA, Itskovitz-Eldor J & Thomson JA 2000 Clonally derived human embryonic stem cell lines maintain pluripotency and proliferative potential for prolonged periods of culture. Developmental Biology 227 271–278. Amit M, Margulets V, Segev H, Shariki K, Laevsky I, Coleman R & Itskovitz-Eldor J 2003 Human feeder layers for human embryonic stem cells. Biology of Reproduction 68 2150–2156. Amit M, Shariki C, Margulets V & Itskovitz-Eldor J 2004 Feeder layerand serum-free culture of human embryonic stem cells. Biology of Reproduction 70 837–845. Anderson DG, Levenberg S & Langer R 2004 Nanoliter-scale synthesis of arrayed biomaterials and application to human embryonic stem cells. Nature Biotechnology 22 863–866. Andrews PW, Benvenisty N, McKay R, Pera MF, Rossant J, Semb H & Stacey GN 2005 The international stem cell initiative: toward benchmarks for human embryonic stem cell research. Nature Biotechnology 23 795–797. Armstrong L, Hughes O, Yung S, Hyslop L, Stewart R, Wappler I, Peters H, Walter T, Stojkovic P, Evans J et al. 2006 The role of PI3K/AKT, MAPK/ERK and NFkappabeta signalling in the maintenance of human embryonic stem cell pluripotency and viability highlighted by transcriptional profiling and functional analysis. Human Molecular Genetics 15 1894–1913. Beattie GM, Lopez AD, Bucay N, Hinton A, Firpo MT, King CC & Hayek A 2005 Activin A maintains pluripotency of human embryonic stem cells in the absence of feeder layers. Stem Cells 23 489–495. Carpenter MK, Rosler E & Rao MS 2003 Characterization and differentiation of human embryonic stem cells. Cloning and Stem Cells 5 79–88. Chen H, Qian K, Hu J, Liu D, Lu W, Yang Y, Wang D, Yan H, Zhang S & Zhu G 2005 The derivation of two additional human embryonic stem cell lines from day 3 embryos with low morphological scores. Human Reproduction 20 2201–2206. Cheng L, Hammond H, Ye Z, Zhan X & Dravid G 2003 Human adult marrow cells support prolonged e...
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This document was uploaded on 09/21/2013.

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