chem research paper 1

By the two equations below where e is the protein

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Unformatted text preview: y the two equations below, where E is the protein shuffle residue and Γ is the ligand vii: CO2 + EZnOH Γ ≡ EZnHCO Γ ≡ EZnH O + HCO Γ 3 Equation 2 2 3 Equation 1vii The first stage is the conversion of the CO2 into bicarbonate via a nucleophilic attack on the zinc bound hydroxide as shown in Equation 1. The CO2 binds in the hydrophobic region and the zinc displaces the bicarbonate ion. The second stage in Equation 2 is the transfer of the proton from the zinc bound water to a proton acceptor [B] to create the zinc hydroxide complex. The schematic of this above reaction is shown in Figure 1. The proton is therefore transferred through the base through a series of hydrogen bonded water molecules.viii _` ! ! 1 = LA !_Z U*The C = !A Q @@Bvalue!B @ the<steady-state = G !D !constant!for the CA is at the diffusionK @ a ! F<@ maximal C = E!D < !\ = FZ 5? A T= @ @<C rateHFC C 5>= !M G BF @A > E BD H of E D ? !G F E > BI > 5 ' ( O <@ = >B>B Q D !FC C 5>= != J D J @!CELFI@Q == !C B FWD !B @ = FZ D E? ! !& !HA ! @ ! H B I > E I > ! D < I F BC ! H < !\ E M L <5? A T= @ E!' . 9N E? !>LM@L@BH A C = E!D !/ ' . ` Z!Q D LFB!&<@ D ? !D !C > X E ! D G BD H A? O ! H IID = K >B for the! encounter D D the !\ = FZ D E !S C @! D L H A D E! @ < A @! ! BA E ML B substrate, controlledSlimit Qi >j !=>!B @BCE>H ADHC!QAofE!HDGenzyme?and AB !M H@! which is 108 M-1s-1.vii The >D LBD !B !A K E@C @ <@ E== I!>B B !D !B @ E\ 5G @ E? !Q D @? !S =<!CE@ ! I = E D @ @ A B !B != BC C @ H < !@ !C AF @ B ! S F5FI@ HFC C 5>= O 1 = LA !G C @ 5!* =J @G C !) O O!! !D ! B I > !i K @ ? !M I A E !$ j limiting step of the reaction is the proton transfer between EZnH2O and EZnOH. In this &<@ = <BME? = K D !B @ = F!= !C >D = B ? !B !>@D ? Z <@I!I= C ? >!6 IE!b9N IL!__gN !BK ! = E ! H < !\ E > > F C @ D F E > I K E !6 ! C p!( >Q 9; ; !C D K S =<!CELG M Amost useful L@Nproviding?anQ bgO B<C ! E? rocess the zinc ion is !D !C D C = !A >= by!2 <@ ` N a N E !&Aactivated ! IE! B ! @ H A G BF @ ? > !b !b !C ! !# ! > solvent molecule for the M @!>LK @B ? !B C !B @@ @= L@!>@J @ D Q @ A@BC ? !>B MI= @ <@ = @B/ = !I= C ? >! @E K > @ < B < > !A > ? > A !B ! A D = E ! E C = \ !B !? A F ! > K E nucleophilic attack and stabilizing the negative change of the transition state. i ' D !@!C O : : : M!" = H A@!C O bba j O <@ @ D C !D !B @ @C != !C <= J @ !M !F@B = ! T B I !9 N @@! B I !_ !& !A G J I H < !G B I > F @ ? 5 AC E F<@C = KC @B!A >LIBEK =idealD E\ 5G @ =<!EDFC C 5BF!C BJ =5O due FBJ =5!FC !M ! I BE ! KZinc is=an E!C !C @ E> @ ! E Q element at anBactive= B !&<= !C to its high !S B ! I = F site > = B E @ affinity and slow A FD @@!LQ E!C ? == E!D !\ = F!i / LEB@!C O bggN = ? >W K C ? !7 C G >Bs G !_bh9j O ! @ J A ? D ? BD H E ! B I !_ !- E D ! E IA !( dissociation c...
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