This preview shows page 1. Sign up to view the full content.
Unformatted text preview: Bulletin: General Guide for
Cryogenically Storing Animal Cell Cultures (Ref. 9). Buying And Borrowing
An alternative to establishing cultures by primary culture is to buy established cell cultures
from organizations such as the ATCC (www.atcc.org), or the Coriell Institute for Medical
Research (ccr.coriell.org). These two nonprofit organizations provide high quality cell lines
that are carefully tested to ensure the authenticity of the cells. More frequently, researchers will obtain (borrow) cell lines from other laboratories. While
this practice is widespread, it has one major drawback. There is a high probability that the
cells obtained in this manner will not be healthy, useful cultures. This is usually due to previous mix-ups or contamination with other cell lines, or the result of contamination with
microorganisms such as mycoplasmas, bacteria, fungi or yeast. These problems are covered
in detail in a Corning Technical Bulletin: Understanding and Managing Cell Culture
Contamination (Ref. 7). What Are Cultured Cells Like? Corning culture dishes are
available in a variety of sizes
and shapes for growing
anchorage-dependent cells. Once in culture, cells exhibit a wide range of behaviors, characteristics and shapes. Some
of the more common ones are described below. John Paul discusses these issues in detail in
Chapter 3 of Cell and Tissue Culture (Ref. 3). Cell Culture Systems
Two basic culture systems are used for growing cells. These are based primarily upon the
ability of the cells to either grow attached to a glass or treated plastic substrate (Monolayer
Culture Sytems) or floating free in the culture medium (Suspension Culture Systems).
Monolayer cultures are usually grown in tissue culture treated dishes, T-flasks, roller bottles,
CellSTACK® Culture Chambers, or multiple well plates, the choice being based on the number of cells needed, the nature of the culture environment, cost and personal preference.
Suspension cultures are usually grown either: Corning culture flasks are
used for growing anchoragedependent cells. 1. In magnetically rotated spinner flasks or shaken Erlenmeyer flasks where the cells are
kept actively suspended in the medium;
2. In stationary culture vessels such as T-flasks and bottles where, although the cells are not
kept agitated, they are unable to attach firmly to the substrate.
Many cell lines, especially those derived from normal tissues, are considered to be
Anchorage-Dependent, that is, they can only grow when attached to a suitable substrate.
Some cell lines that are no longer considered normal (frequently designated as Transformed
Cells) are frequently able to grow either attached to a substrate or floating free in suspension;
they are Anchorage-Independent. In addition, some normal cells, such as those found in
the blood, do not normally attach to substrates and always grow in suspension. Types of Cells
Cultured cells are usually described based on their morphology (shape and appearance) or
their functional characte...
View Full Document
This note was uploaded on 09/30/2013 for the course PHARM 101 taught by Professor Mishra during the Fall '11 term at Birla Institute of Technology & Science, Pilani - Hyderabad.
- Fall '11