intro_animal_cell_culture - Introduction to Animal Cell...

Info icon This preview shows pages 1–3. Sign up to view the full content.

View Full Document Right Arrow Icon
John A. Ryan, Ph.D. Corning Incorporated Life Sciences 900 Chelmsford St. Lowell, MA 01851 Table of Contents Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 What is Cell and Tissue Culture? . . . . . . . . . . . . . . . . . . 1 How are Cell Cultures Obtained? . . . . . . . . . . . . . . . . . . 2 What Are Cultured Cells Like? . . . . . . . . . . . . . . . . . . . . 3 What Are Some of the Problems Faced by Cultured Cells? . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 How to Decide if Cultured Cells Are “Happy”? . . . . . . . 6 What is Cell Culture Used For? . . . . . . . . . . . . . . . . . . . 6 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 Introduction Cell culture has become one of the major tools used in the life sciences today. This guide is designed to serve as a basic introduction to animal cell culture. It is appropriate for lab- oratory workers who are using it for the first time, as well as for those who interact with cell culture researchers and who want a better understanding of the key concepts and termi- nology in this interesting and rapidly growing field. What is Cell and Tissue Culture? Tissue Culture is the general term for the removal of cells, tissues, or organs from an animal or plant and their subse- quent placement into an artificial environment conducive to growth. This environment usually consists of a suitable glass or plastic culture vessel containing a liquid or semi- solid medium that supplies the nutrients essential for sur- vival and growth. The culture of whole organs or intact organ fragments with the intent of studying their continued function or development is called Organ Culture . When the cells are removed from the organ fragments prior to, or during cultivation, thus disrupting their normal relation- ships with neighboring cells, it is called Cell Culture . Although animal cell culture was first successfully undertak- en by Ross Harrison in 1907, it was not until the late 1940’s to early 1950’s that several developments occurred that made cell culture widely available as a tool for scientists. First, there was the development of antibiotics that made it easier to avoid many of the contamination problems that plagued earlier cell culture attempts. Second was the development of Introduction to Animal Cell Culture Technical Bulletin
Image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
2 the techniques, such as the use of trypsin to remove cells from culture vessels, necessary to obtain continuously growing cell lines (such as HeLa cells). Third, using these cell lines, scientists were able to develop standardized, chemically defined culture media that made it far easier to grow cells. These three areas combined to allow many more scientists to use cell, tissue and organ culture in their research. During the 1960’s and 1970’s, commercialization of this technology had further impact on cell culture that continues to this day. Companies, such as Corning, began to develop and sell disposable plastic and glass cell culture products, improved filtration products and mate- rials, liquid and powdered tissue culture media, and laminar flow hoods. The overall result of these and other continuing technological developments has been a widespread increase in the number of laboratories and industries using cell culture today.
Image of page 2
Image of page 3
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern