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Bio 321 F13 lecture 6 for post

Produced no band produced heterozygote for the band

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Unformatted text preview: typically dinucleo1de (e.g. ATATATATAT) or trinucleo1de (e.g. ATCATCATCATC) •  amplify using primers complementary to non- repe11ve, non- variable flanking sequences –  have to know the flanking sequences •  a lot of work finding microsat primer sets that work •  alleles are defined as number of repeats –  from above (AT)5, (ATC)4 –  or by overall size of fragment microsatellite data are visualized using capillary electrophoresis individuals molecular weight alleles shown in different colors http://www.bio.davidson.edu/courses/ genomics/method/micro3.gif Microsatellites are co-dominant markers =can tell homozygotes and heterozygotes for all alleles • microsatellite loci have higher mutations rates (in terms of changes in repeat number) than other loci (in terms of nucleotide substitutions) •  for this reason they are often variable between individuals within populations SNPs single nucleo1de polymorphisms •  arose from analysis of DNA sequence data –  but actually RFLP, RAPD, and AFLP polymorphisms are SNPs for the most part •  can be detected using various sequencing technologies or by designing specific PCR primers •  4 alleles possible (A,G,C,T), typically a popula1on will only have two alleles (e.g. A,G) –  in D. melanogaster and H. sapiens this ouen suggests admixture of two popula1ons during recent evolu1onary history SNP data SNP data thursday •  •  •  •  non- random ma1ng inbreeding heterozygosity exercise...
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