1 an overview of yeast in the laboratory a b

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Unformatted text preview: ge 3 A.1 An Overview of Yeast in the Laboratory (a) (b) Subtelomeric repeats 2060–3060 1522 1090 1091 948 924 813 784 745 666 575 562 439 315 270 230 Centromere 30–40 kb 3 Origins of replications Telomeres XII IV VII XV XVI XIII II XIV X XI V VIII IX III VI I Figure A.3 Yeast chromosomes. (a) Elements of a chromosome. Each linear chromosome contains one centromere, multiple origins of replication, subtelomeric repeats, and telomeres at the ends of the linear DNA. (b) Molecular karyotype of yeast chromosomes. Using pulse-field gel electrophoresis, the chromosomes can be separated by size. according to the same blueprint, with one centromere/ kinetochore that binds to one microtubule, two termini consisting of longer subtelomeric repeats followed by short telomere repeats at the very ends, and multiple origins of replication spaced approximately 30–40 kb apart (of which only a small subset are used to initiate replication at any one time) (Fig. A.3a). Yeast chromosomes are packaged into nucleosomes consisting of the core histones H2A, H2B, H3,...
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This note was uploaded on 10/03/2013 for the course BI 206 taught by Professor Celenza during the Spring '08 term at BU.

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