Saccharomyces_A

1 an overview of yeast in the laboratory and the loss

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Unformatted text preview: 1 An Overview of Yeast in the Laboratory and the loss the function is in C 1 C 2 , the loss of function would be the phenotype expressed. Another powerful genetic technique easily exploited in yeast is the isolation of mutants in which a mutant phenotype is suppressed. Suppressor mutations can be either second mutations in a gene that restore the function of the encoded protein or mutations in genes whose products interact physically or in pathways that function as backup pathways able to buffer the defect in the first gene. In summary, the power of yeast genetics includes the ability to 7 DNA containing cloned KANR gene DNA containing cloned yeast gene to be disrupted KANR Remove most of the gene to be disrupted and insert KANR gene by recombinant DNA technology. Transform linear DNA fragment into diploid KANS yeast. Select KANR cells. Fragment recombines with one chromosome and replaces wild-type gene with disrupted gene. • • • • isolate large numbers of mutants, even rare mutants; easily characterize dominance and recessiveness; isolate mutants with defects in essential genes; order gene products in a pathway using epistasis testing; • identify interacting gene products via suppressor analysis Diploid heterozygous for disruption Transformation and Integration of DNA Are Very Efficient Sporulation Ascus with four haplo...
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