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Unformatted text preview: 1 An Overview of Yeast in the Laboratory and the loss the function is in C 1 C 2 , the loss of function
would be the phenotype expressed.
Another powerful genetic technique easily exploited in
yeast is the isolation of mutants in which a mutant phenotype is suppressed. Suppressor mutations can be either second mutations in a gene that restore the function of the
encoded protein or mutations in genes whose products interact physically or in pathways that function as backup
pathways able to buffer the defect in the ﬁrst gene.
In summary, the power of yeast genetics includes the
ability to 7 DNA containing
cloned KANR gene DNA containing cloned
yeast gene to be disrupted KANR
Remove most of the gene to be
disrupted and insert KANR gene
by recombinant DNA technology. Transform linear DNA fragment into
diploid KANS yeast. Select KANR cells.
Fragment recombines with one chromosome
and replaces wild-type gene with disrupted gene. •
• isolate large numbers of mutants, even rare mutants;
easily characterize dominance and recessiveness;
isolate mutants with defects in essential genes;
order gene products in a pathway using epistasis
• identify interacting gene products via suppressor
analysis Diploid heterozygous for disruption Transformation and Integration
of DNA Are Very Efﬁcient Sporulation Ascus with four haplo...
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- Spring '08