A third genomic approach consists in the identication

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Unformatted text preview: onal response to pheromone treatment depends on Ste12 because the changes in expression of all of the genes that respond to pheromone are abolished by loss of Ste12 function. A third genomic approach consists in the identification of Ste12-binding sites across the genome. Transcription factors like Ste12 activate transcription by binding to regulatory sequences upstream of the 5 ¿ end of regulated transcripts. To map the genomic locations for Ste12 binding, cells were treated to chemically cross-link DNA to proteins and then broken open. Enrichment for Ste12-DNA har06584_refA_001-020 11/4/06 10:10 AM Page 15 A.3 Mating: Cell-to-Cell Communication Through Pheromones 15 (c) Mapping of Ste12 DNA binding sites. (a) Genomic profile of yeast grown in the presence of α pheromone. Binding site 2 Increased expression 1 No change 0 Decreased expression –1 –2 –1 0 Intensity 1 (b) Genomic profile of a Ste12 – mutant. 2 Increased expression 1 IP-enriched No change Unenriched DNA 0 Merged Decreased expression –1 –1 0 1 2 Intensity Figure A.16 DNA array analysis of the pheromone response. (...
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This note was uploaded on 10/03/2013 for the course BI 206 taught by Professor Celenza during the Spring '08 term at BU.

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