Unformatted text preview: c. 3 4 5 6 7 4 5 6 7 2
0 b. Dig1 and Dig2 proteins are both required for inhibition of Ste12 activity. A dig1 dig2 double
mutant has a constitutive mating defect. Based
on the pheromone response pathway, why do
you think this is true?
A dig1 dig2 double mutant also exhibits invasive growth on solid media. What phenotype do
you think you would see in a dig1 dig2
ste12 triple mutant? A-11 Six genes, each conferring the ability to use the sugar 3 4 5 6 7 3 4 5 6 7 Are these results derived from a complementation
or from a recombination experiment?
The a / a diploids from the experiment in part a are
now induced to sporulate. Out of 400 spores examined from each a / a diploid, the numbers that
were able to grow on minimal medium are scored
0 d. The biochemical pathway involved in the biosynthesis of sneezan is linear. One of the steps of the
pathway is catalyzed by a heterodimeric enzyme,
with each subunit encoded by a different gene.
What is the biochemical pathway? Identify the
genes that encode the enzyme involved in each
step. Indicate which enzyme is dimeric.
A-10 a. 3 19 3
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- Spring '08
- DNA, MAT locus, Yeast Geneticist