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Unformatted text preview: a) Fluorescently labeled cDNAs derived from wild-type cells that were
untreated or treated with pheromone were hybridized to spotted DNA arrays representing over 6000 ORFs. Data are displayed as intensity versus red:green ratio for each ORF. Grey dots represent genes that show no change in the level of gene expression. Red or green
dots represent genes that were induced or repressed, respectively. (b) cDNAs derived from a wild-type strain and a ste12 mutant strain
were hybridized to the arrays. Grey dots represent genes whose expression was unchanged. Green dots represent mRNAs whose expression was shown to change in (a). (c) The ﬁgure shows a close-up of a scanned image of a DNA chip containing DNA fragments representing over 6000 intergenic regions of the yeast genome. The arrow points to a spot where the red intensity is overrepresented, identifying
a region bound in vivo by the Ste12 protein. complexes was accomplished by immunoprecipitation
using an antibody that recognizes and binds to Ste12. The
DNA in the enriche...
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This note was uploaded on 10/03/2013 for the course BI 206 taught by Professor Celenza during the Spring '08 term at BU.
- Spring '08