Saccharomyces_A

Expression of the suc2 gene was examined in the wild

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Unformatted text preview: nd maltose as well as sucrose. The common feature of the affected genes is that all are glucose repressible (not expressed when glucose is present). Expression of the SUC2 gene was examined in the wild type and the mutant under repressing and nonrepressed conditions. How would you describe the transcription pattern? Wild type – Glucose +Glucose snf1 – Glucose +Glucose har06584_refA_001-020 20 11/13/06 09:12 PM Page 20 Reference A Saccharomyces cerevisiae: Genetic Portrait of a Yeast A-12 To study DNA repair mechanisms, researchers iso- lated yeast mutants that were sensitive to various types of radiation. For example, they isolated mutants that were more sensitive to damage by UV light (and therefore died at a lower dose of UV radiation). Ten haploid mutants were isolated. a. How would you do the complementation tests for these mutants in yeast? b. Based on the following complementation chart, how many genetic loci are represented by these mutants? 1 1 2 3 4 5 6 2 3 4 5 6 c. Double mutants were constructed containing some of the UV-sensitive mutations. I...
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