Saccharomyces_A

For the following questions assume that you have

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Unformatted text preview: see if there is variation in the size of the rDNA repeat in the clones? A-4 Expression of genes involved in the same process (for example, amino-acid biosynthesis) is often coregulated in yeast. For the following questions, assume that you have cloned the genes involved in histidine metabolism. a. How could you show if the HIS genes are transcriptionally coregulated? b. If there is transcriptional coregulation, what might you expect to see in the sequence in the regulatory region of these genes and why? A-5 Several copies of subtelomeric repeats may be present at the ends of chromosomes, resulting in more than 20 kb of repeat DNA. a. How could you show which chromosomes contain repeated DNA sequences near the ends of each chromosome? b. If you had a genomic library of plasmid clones with an average insert size of 15 kb and were trying to make a physical map, how would you know which chromosome your cloned DNA came from? A-6 In screening a population of diploid yeast for a defect in chromosome segregation, you found that the c...
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