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Unformatted text preview: see if there is
variation in the size of the rDNA repeat in the clones?
A-4 Expression of genes involved in the same process (for example, amino-acid biosynthesis) is often coregulated in yeast. For the following questions, assume
that you have cloned the genes involved in histidine
a. How could you show if the HIS genes are transcriptionally coregulated?
b. If there is transcriptional coregulation, what
might you expect to see in the sequence in the
regulatory region of these genes and why? A-5 Several copies of subtelomeric repeats may be present at the ends of chromosomes, resulting in more than 20
kb of repeat DNA.
a. How could you show which chromosomes contain repeated DNA sequences near the ends of
b. If you had a genomic library of plasmid clones
with an average insert size of 15 kb and were
trying to make a physical map, how would you
know which chromosome your cloned DNA came
A-6 In screening a population of diploid yeast for a defect in chromosome segregation, you found that the c...
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- Spring '08