Saccharomyces_A

How many complementation groups involved in the

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Unformatted text preview: 0 4 100 3 100 0 5 100 3 100 0 0 6 100 0 100 3 3 0 7 5 100 0 100 100 100 0 How can you explain the two classes of results here (100 spores or 5 2 ? How many complementation groups involved in the biosynthesis of sneezan are represented in this collection of seven mutations? Which genes are in which complementation groups? sucrose have been mapped to different loci in different Saccharomyces strains. a. You have cloned the SUC2 gene from a S. cerevisiae strain. How can you tell if the SUC loci in other strains contain DNA homologous to that at the SUC2 locus? b. Other Suc strains have DNA homologous to the SUC2 gene but, in some cases, there is not enough information for full gene function. What would you call these partial genes? c. Starting with an SUC2 strain, you have identified cells incapable of fermenting sucrose. Some of the mutants are defective in the gene at the SUC2 locus (Suc2 ). Other Suc strains are pleiotropic. These genes, called snf (sucrose nonfermenting) genes, affect expression of galactose a...
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This note was uploaded on 10/03/2013 for the course BI 206 taught by Professor Celenza during the Spring '08 term at BU.

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