Saccharomyces_A

How many different repair pathways do these mutations

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Unformatted text preview: f the UV sensitivity of the double mutant is greater than that of the individual mutants, the mutations are in different repair pathways. How many different repair pathways do these mutations represent? What mutations are in each pathway? Single mutant 1 3 6 Sensitivity to UV Double mutant 1,3 1,6 3,6 d. Assume you have cloned the genes and now want to ask whether genes identified in these mutant searches are expressed constitutively in cells or only in response to damage. How would you design your experiment? e. To identify additional genes that might be responding to DNA damage, you have decided to construct a library of yeast DNA fused to the lacZ gene. How would you use this library to search for more genes that are induced by DNAdamaging agents such as radiation?...
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This note was uploaded on 10/03/2013 for the course BI 206 taught by Professor Celenza during the Spring '08 term at BU.

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