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Unformatted text preview: f the UV
sensitivity of the double mutant is greater than
that of the individual mutants, the mutations are
in different repair pathways. How many different repair pathways do these mutations represent? What mutations are in each pathway?
6 Sensitivity to UV Double mutant
3,6 d. Assume you have cloned the genes and now
want to ask whether genes identiﬁed in these
mutant searches are expressed constitutively in
cells or only in response to damage. How would
you design your experiment?
e. To identify additional genes that might be responding to DNA damage, you have decided to
construct a library of yeast DNA fused to the
lacZ gene. How would you use this library to
search for more genes that are induced by DNAdamaging agents such as radiation?...
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This note was uploaded on 10/03/2013 for the course BI 206 taught by Professor Celenza during the Spring '08 term at BU.
- Spring '08