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If there is a gain of function mutation c in gene c

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Unformatted text preview: -> C -> D). If there is a gain-of-function mutation C* in gene C such that the protein is always active, that active phenotype would be seen even if it was combined in a cell with a loss-of-function mutation in A (A ). In this case, C is epistatic to A and must be after A in the pathway. If the gain-of-function mutation is in A (A*) (b) Mutation in an Essential Gene Diploid yeast cell Diploid yeast cell Mutagenize Mutagenize Mutation in one gene (URA3) Mutation in DNA Replication Gene Meiosis (Sporulation) Meiosis (Sporulation) Dead URA3 URA3 URA3 Dead URA3 2 Ura : 2 Ura spores 2 live spores Figure A.7 Mutant isolation in a diploid cell. (a) A mutation on one homolog produces 2 and 2 spores if the mutation is recessive and is in a non-essential gene. (Only one pair of homologous chromosomes is shown in the figure for simplicity.) (b) If the mutation occurs in a gene that is essential for growth, only two live spores are recovered after meiosis. har06584_refA_001-020 11/4/06 10:09 AM Page 7 A....
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