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Unformatted text preview: enetic Portrait of a Yeast pheromone; normal a cells repress expression of these
genes. MATa/ MAT cells normally repress transcription of
haploid-speciﬁc genes; however, loss of a1 or 2 function
in these cells results in transcription of the haploid-speciﬁc
genes, including genes that inﬂuence the pheromone response, cell fusion, and meiosis. The Mcml Protein Acts as a Positive and
Studies of the binding to DNA of isolated 1 and 2
proteins have shown that the transcriptional activation of
-speciﬁc genes in an a cell requires the 1 gene product
together with a protein known as Mcm1. In this same
cell, the DNA binding of the 2 gene product together
with the Mcm1 protein represses the transcription of aspeciﬁc genes (Fig. A.10a). By contrast, the transcriptional
activation of a-speciﬁc genes depends only on Mcm1, requiring no product encoded by the MAT locus (Fig. A.10b).
The Mcm1 protein thus functions as a transcription factor
in both a and cells but actually has opposite effects in the
two haploid cell types. This single transcriptio...
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This note was uploaded on 10/03/2013 for the course BI 206 taught by Professor Celenza during the Spring '08 term at BU.
- Spring '08