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Once the yeast genome sequence became available it

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Unformatted text preview: l-to-cell communication. Once the yeast genome sequence became available, it was possible to create deletion mutations in each of the over 6000 ORFs and test the effects of the mutations. Deletion mutants were generated by PCR synthesis of fragments containing 25 bases from the beginning and end of each ORF, with a KANR gene acting as a selectable marker between the beginning and ending pieces of the gene. After transformation of these linear fragments into cells, the DNA integrated by homologous recombination. This very valuable collection of deletion mutants is now available to all researchers, who can use it to search for mutations in their favorite process. In one study, researchers assayed the effect of each deletion mutation (null mutation) in both haploids and diploids. From this analysis, they found that 80% of yeast genes appeared to be non-essential. How can yeast tolerate so many individual deletions? One hypothesis is that there is redundancy of function in the genome, with gene products that work in different pa...
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