Saccharomyces_A

Researchers used dna arrays see chapter 10 of the

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Unformatted text preview: pe of signal transduction pathway dissected in the New genome-wide assays have identified additional genes that are turned on in response to pheromones. Researchers used DNA arrays (see Chapter 10 of the main textbook) to assay simultaneously the abundance of cDNA’s corresponding to virtually all cellular mRNAs in cells before and after treatment with pheromone (Fig. A.16a). The arrays contain oligonucleotides corresponding to all the annotated ORFs known to code for yeast proteins (about 6000 of them). In a representative experiment, cDNA pools from two sets of a cells—one treated with a pheromone, the other untreated—were differentially labeled with the fluorescent dyes Cy3 (green) or Cy5 (red) and then mixed and probed by competitive hybridization to the genomic arrays. Changes in the abundance of an individual mRNA produces a spot that fluoresces red or green, depending on whether the corresponding mRNA increases or decreases in abundance in response to -pheromone treatment. A genomic view of the data can be obtained b...
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