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Unformatted text preview: d sample was then ampliﬁed by PCR
and labeled with the ﬂuorescent dye Cy5 (red). A sample
that was not enriched by immunoprecipitation was ampliﬁed and labeled with Cy3 (green). The DNA samples were
hybridized to DNA arrays containing oligonucleotides corresponding to over 6000 intergenic DNA sequences (the
spaces between genes). When the Cy5 and Cy3 signals
were merged, spots corresponding to sequences that bound
to Ste12 showed red ﬂuorescence, whereas those that did
not bind Ste12 were yellow or green (Fig. A.16c). Twentynine genes were identiﬁed whose transcription is controlled through the binding of Ste12 to the corresponding
5 ¿ regulatory regions. The list includes a number of genes
shown in Figure A.15, including FUS3 (MAP kinase), FAR1
1 G1 cell-cycle arrest), FUS1 (cell fusion), and KAR5 (nuclear
fusion). Ste12 is therefore a global transcriptional regulator, similar to the CRP protein in E. coli that binds to and regulates many genes in response to an environmental cue.
Two ﬁndings from these genomic studies indicate that
additional circuitry exists in the pheromone response pathway downstream of Ste12. First, Ste12 is an activator of
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This note was uploaded on 10/03/2013 for the course BI 206 taught by Professor Celenza during the Spring '08 term at BU.
- Spring '08