Saccharomyces_A

The dna samples were hybridized to dna arrays

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Unformatted text preview: d sample was then amplified by PCR and labeled with the fluorescent dye Cy5 (red). A sample that was not enriched by immunoprecipitation was amplified and labeled with Cy3 (green). The DNA samples were hybridized to DNA arrays containing oligonucleotides corresponding to over 6000 intergenic DNA sequences (the spaces between genes). When the Cy5 and Cy3 signals were merged, spots corresponding to sequences that bound to Ste12 showed red fluorescence, whereas those that did not bind Ste12 were yellow or green (Fig. A.16c). Twentynine genes were identified whose transcription is controlled through the binding of Ste12 to the corresponding 5 ¿ regulatory regions. The list includes a number of genes shown in Figure A.15, including FUS3 (MAP kinase), FAR1 1 G1 cell-cycle arrest), FUS1 (cell fusion), and KAR5 (nuclear fusion). Ste12 is therefore a global transcriptional regulator, similar to the CRP protein in E. coli that binds to and regulates many genes in response to an environmental cue. Two findings from these genomic studies indicate that additional circuitry exists in the pheromone response pathway downstream of Ste12. First, Ste12 is an activator of transcription...
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This note was uploaded on 10/03/2013 for the course BI 206 taught by Professor Celenza during the Spring '08 term at BU.

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