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Unformatted text preview: y plotting the intensity of the signal for each mRNA versus the expression
ratio (that is, expression in -pheromone treated cells versus expression in untreated cells). The data showed that
more than 200 genes increase expression and nearly as
many decrease expression as a result of -pheromone treatment. In agreement with earlier genetic analyses, genes required for all stages of the mating response were
represented among those whose expression increased. pheromone treatment repressed the expression of genes required for cell-cycle progression, DNA replication,
budding, and mitosis. Similar results were found when
cells were treated with a pheromone.
Another way to study the response of cells to
pheromone treatment is to assay expression from strains
that carry speciﬁc defects in genes known to be involved in
the response. Figure A.16b shows a genomic expression
proﬁle generated in the same way as the one described previously except that the response of two different a strains
were compared, a wild-type strain and one carrying a deletion of the gene coding for the Ste12 transcription factor.
The experiment shows that the entire transcripti...
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This note was uploaded on 10/03/2013 for the course BI 206 taught by Professor Celenza during the Spring '08 term at BU.
- Spring '08