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Unformatted text preview: causing the conversion of G -GDP to
G -GTP and this conversion, in turn, causing the dissociation of the G / G subunits from the heterotrimeric
G-protein complex. The dissociated but still membranebound G / G dimeric complex initiates a signal that propagates through the series of proteins in the signal transduction
pathway. The pathway ultimately leads to the transcriptional
activation of genes whose products are required for G1 cellcycle arrest, cell fusion, and nuclear fusion. Inhibition of a cell
growth by α pheromone
Wild-type α cells Sterile α mutant
unable to make α pheromone Inhibition
of α cell growth
by a pheromone Sterile α mutant
unable to respond to
a pheromone Sterile a mutant
unable to respond to α pheromone Wild-type
Sterile a mutant
unable to make
a pheromone Figure A.14 Mutants defective in producing or responding to mating signals. Strains can be cross-streaked on plates to test for
defects in sending or receiving pheromone messages. Pheromones from a strain of one mating type bind to a strain of the opposite mating type...
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This note was uploaded on 10/03/2013 for the course BI 206 taught by Professor Celenza during the Spring '08 term at BU.
- Spring '08