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Unformatted text preview: d
recessive alleles, and the segregation of alleles during
a. Because the newly isolated mutations are present
in haploid strains you will not know immediately
if they are dominant or recessive. To determine if a
mutation is dominant or recessive, mate the mutant haploid strain with a wild-type haploid
strain. If the diploid cannot grow without adenine
in the medium, the mutation is dominant. If the
diploid cells have the wild-type phenotype, the
adenine mutation is recessive.
b. If the mutation is dominant, you can determine its
effects on sporulation using the diploid strain constructed for part a. If the mutation is recessive, you
would have to construct a homozygous recessive
diploid. You could do this by switching the mating
type of the original strain to obtain an a haploid
cell and mating this with the original a haploid
II. You have cloned a gene encoding the histone subunit H2A.
a. Now you want to do a gene replacement with a
nonfunctional copy of the gene and observe the
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This note was uploaded on 10/03/2013 for the course BI 206 taught by Professor Celenza during the Spring '08 term at BU.
- Spring '08