Saccharomyces_A

To determine if a mutation is dominant or recessive

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Unformatted text preview: d recessive alleles, and the segregation of alleles during meiosis. a. Because the newly isolated mutations are present in haploid strains you will not know immediately if they are dominant or recessive. To determine if a mutation is dominant or recessive, mate the mutant haploid strain with a wild-type haploid strain. If the diploid cannot grow without adenine in the medium, the mutation is dominant. If the diploid cells have the wild-type phenotype, the adenine mutation is recessive. b. If the mutation is dominant, you can determine its effects on sporulation using the diploid strain constructed for part a. If the mutation is recessive, you would have to construct a homozygous recessive diploid. You could do this by switching the mating type of the original strain to obtain an a haploid cell and mating this with the original a haploid cell. II. You have cloned a gene encoding the histone subunit H2A. a. Now you want to do a gene replacement with a nonfunctional copy of the gene and observe the phenotype o...
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This note was uploaded on 10/03/2013 for the course BI 206 taught by Professor Celenza during the Spring '08 term at BU.

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