To test this hypothesis researchers looked for

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Unformatted text preview: thways acting as backup. To test this hypothesis, researchers looked for combinations of mutations that would lead to lethality (synthetic lethality; see Chapter 20 of the main textbook). They interrogated the collection of deletion mutants by crossing them with loss-of-function alleles in known genes involved in processes such as cell wall biosynthesis and DNA repair. Each loss-of-function query gene showed lethality with 1-146 other genes, with the average being 34 genes. This result indicated that several genes must buffer the effects of a single null mutation. Many of the synthetic lethal mutations were in genes that have fundamentally similar functions although their primary roles are in different pathways. This analysis allowed researchers to begin mapping the network of interactions between genes in essential processes that are common to all eukaryotes. In summary, yeast continues to be a wonderful model eukaryote in which to explore basic biological pathways for the following reasons. • Basic cell processes are conse...
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This note was uploaded on 10/03/2013 for the course BI 206 taught by Professor Celenza during the Spring '08 term at BU.

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