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Unformatted text preview: thways acting as backup. To test this hypothesis, researchers
looked for combinations of mutations that would lead to
lethality (synthetic lethality; see Chapter 20 of the main
textbook). They interrogated the collection of deletion mutants by crossing them with loss-of-function alleles in known
genes involved in processes such as cell wall biosynthesis and DNA repair. Each loss-of-function query gene showed
lethality with 1-146 other genes, with the average being 34
genes. This result indicated that several genes must buffer
the effects of a single null mutation. Many of the synthetic
lethal mutations were in genes that have fundamentally
similar functions although their primary roles are in different pathways. This analysis allowed researchers to begin
mapping the network of interactions between genes in essential processes that are common to all eukaryotes.
In summary, yeast continues to be a wonderful model
eukaryote in which to explore basic biological pathways
for the following reasons.
• Basic cell processes are conse...
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This note was uploaded on 10/03/2013 for the course BI 206 taught by Professor Celenza during the Spring '08 term at BU.
- Spring '08