Unformatted text preview: id cells.
Two have the disrupted gene
containing the selectable marker. Yeast cells are susceptible to efﬁcient transformation by
linear or circular DNA molecules from external sources. To
execute a transformation experiment, researchers construct
a DNA molecule (plasmid) carrying a marker in the form
of a functional yeast gene or antibiotic resistance gene, and
expose a population of yeast cells to a solution of the plasmids. They then conﬁrm the presence of this DNA in transformed cells by ascertaining whether the marker gene has
supplied a function missing in the transformation recipients, or host cells. For example, a plasmid carrying the
yeast LEU2 gene, coding for an enzyme in leucine biosynthesis, causes a host cell carrying a loss-of-function mutation in the LEU2 gene to become Leu in phenotype. The
very high level of homologous recombination in yeast facilitates integration of the DNA into the chromosome. Separate spores by micromanipulation.
Place on nutrient plate containing the
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- Spring '08
- DNA, MAT locus, Yeast Geneticist