Saccharomyces_A

What is the function of each gene how do gene

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Unformatted text preview: rowth? What is the function of each gene? How do gene products interact? Can we define a complete set of genes regulated by a specific transcription factor? The first microarrays were developed using yeast DNA (see Chapter 10 of the main textbook). The small genome size and lack of introns make it possible to produce yeast arrays that represent all of the ORFs or even the entire genome. To monitor gene expression under different environment conditions, one can isolate RNA from two different conditions and hybridize it to the array. To determine which genes are regulated by a transcription factor, one can compare the expression patterns of a mutant defective in the transcription factor with those of wild-type cells. The ability to generate genome-wide gene expression profiles with DNA arrays is transforming the way we approach genetic analyses and broadening our ability to visualize how all the parts of a cell function together. Examples of these analyses are described in this portrait’s final section on the mating pathway and cel...
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