Unformatted text preview: rowth? What is the function of each gene?
How do gene products interact? Can we deﬁne a complete
set of genes regulated by a speciﬁc transcription factor?
The ﬁrst microarrays were developed using yeast DNA
(see Chapter 10 of the main textbook). The small genome
size and lack of introns make it possible to produce yeast
arrays that represent all of the ORFs or even the entire
genome. To monitor gene expression under different environment conditions, one can isolate RNA from two different conditions and hybridize it to the array. To determine
which genes are regulated by a transcription factor, one can
compare the expression patterns of a mutant defective in
the transcription factor with those of wild-type cells. The
ability to generate genome-wide gene expression proﬁles
with DNA arrays is transforming the way we approach genetic analyses and broadening our ability to visualize how
all the parts of a cell function together. Examples of these
analyses are described in this portrait’s ﬁnal section on the
mating pathway and cel...
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- Spring '08
- DNA, MAT locus, Yeast Geneticist