When the fragment is transformed into a diploid yeast

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Unformatted text preview: e of interest. When the fragment is transformed into a diploid yeast cell, it can recombine with the gene in one of the chromosomes by homology, replacing the good copy with a mutant. The heterozygous diploid, when sporulated, produces two haploid spores containing the disrupted copy and two spores with the normal copy of the gene. har06584_refA_001-020 8 11/4/06 10:09 AM Page 8 Reference A Saccharomyces cerevisiae: Genetic Portrait of a Yeast using a DNA fragment carrying a disrupted gene. The disruption includes a gene encoding resistance to the antibiotic kanamycin (KANR), which serves as the selectable marker. Chromosomal integration of the disrupted gene occurs through the equivalent of a double crossover, and the presence of the mutant gene is detected by the selectable marker. Genome-Wide Analyses The availability of the complete genome sequence, the small genome size, and the many already developed techniques enable yeast geneticists to explore many previously unapproachable questions, such as: How many genes are essential for g...
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This note was uploaded on 10/03/2013 for the course BI 206 taught by Professor Celenza during the Spring '08 term at BU.

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