Saccharomyces_A

A complementation 1 silent copy of mating type

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Unformatted text preview: strains, then the HIR2 and HIR3 gene products are not required for copy number sensitive transcription. Problems A-1 Choose the matching phrase in the right column for each of the terms in the left column. a. complementation 1. silent copy of mating type information b. epistasis 2. expressed copy of mating type information c. homothallic 3. test for order of genes in a pathway d. pheromone 4. test for one versus two genes e. MAT 5. able to switch mating type f. HML g. heterothallic 6. unable to switch mating type 7. chemical signal A-2 The yeast genome is approximately 12,000 kb in length and contains about 6000 open reading frames encoding an average of 500 amino acids. Using these numbers, calculate the proportion of the yeast genome that is protein-coding DNA. A-3 Chromosome XII in yeast is about 2060–3060 kb in length depending on the number of the rDNA repeats (100–200 copies of a 9 kb repeating unit). If you grow up 10 individual yeast cells as clones in liquid culture, how could you analyze each clone to...
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This note was uploaded on 10/03/2013 for the course BI 206 taught by Professor Celenza during the Spring '08 term at BU.

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