Unformatted text preview: ite cell type activates a signal transduction system that results in the transcription of genes whose
products help arrest the cell cycle and contribute to
nuclear and cellular fusion. Internet Resource for S. cerevisiae
Saccharomyces Genome database (genome database with
a great interface to lots of interesting information:
www.yeastgenome.org/ har06584_refA_001-020 11/4/06 10:10 AM Page 17 Solved Problems 17 Solved Problems
I. You want to study metabolism of purines in S. cere- visiae and begin your analysis by isolating auxotrophic
mutants that cannot synthesize adenine. To achieve
this, you mutagenize haploid a cells and screen for
cells that require adenine for growth.
a. Will you know if your mutations are dominant or
recessive? If not, how could you ﬁnd out?
b. If you wanted to know if any of the mutations had
an effect on sporulation, how could you test this?
What strain would you need to construct? Answer:
This problem requires an understanding of the yeast
life cycle, ploidy and the expression of dominant an...
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