Cerevisiae saccharomyces genome database genome

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Unformatted text preview: ite cell type activates a signal transduction system that results in the transcription of genes whose products help arrest the cell cycle and contribute to nuclear and cellular fusion. Internet Resource for S. cerevisiae Saccharomyces Genome database (genome database with a great interface to lots of interesting information: har06584_refA_001-020 11/4/06 10:10 AM Page 17 Solved Problems 17 Solved Problems I. You want to study metabolism of purines in S. cere- visiae and begin your analysis by isolating auxotrophic mutants that cannot synthesize adenine. To achieve this, you mutagenize haploid a cells and screen for cells that require adenine for growth. a. Will you know if your mutations are dominant or recessive? If not, how could you find out? b. If you wanted to know if any of the mutations had an effect on sporulation, how could you test this? What strain would you need to construct? Answer: This problem requires an understanding of the yeast life cycle, ploidy and the expression of dominant an...
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