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Unformatted text preview: c base differences between the two
genes could be used as probes in two separate hybridizations to mRNAs that had been separated by
electrophoresis and transferred to ﬁlter paper
e. If the DNA upstream to (5 ¿ to) the H2A gene was
cloned next to a reporter gene such as lacZ, this
construct could be used to analyze the role of the
DNA in cell-cycle regulation. Different portions of
c. the upstream region could be deleted, the resulting
clones transformed into yeast, and expression (as
measured by production of -galactosidase) analyzed at different times in the cell cycle.
f. Because the lack of functioning protein in the null
mutations led to constitutive expression, HIR2
and HIR3 must encode negative regulators.
g. To determine if HIR2 and HIR3 are also involved
in copy number regulated transcriptional control,
the high copy number histone gene plasmids
should be transformed into hir2 or hir3 mutants. If
the level of expression is repressed to the same extent as in HIR...
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This note was uploaded on 10/03/2013 for the course BI 206 taught by Professor Celenza during the Spring '08 term at BU.
- Spring '08