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E if the dna upstream to 5 to the h2a gene was cloned

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Unformatted text preview: c base differences between the two genes could be used as probes in two separate hybridizations to mRNAs that had been separated by electrophoresis and transferred to filter paper (Northern hybridization). e. If the DNA upstream to (5 ¿ to) the H2A gene was cloned next to a reporter gene such as lacZ, this construct could be used to analyze the role of the DNA in cell-cycle regulation. Different portions of c. the upstream region could be deleted, the resulting clones transformed into yeast, and expression (as measured by production of -galactosidase) analyzed at different times in the cell cycle. f. Because the lack of functioning protein in the null mutations led to constitutive expression, HIR2 and HIR3 must encode negative regulators. g. To determine if HIR2 and HIR3 are also involved in copy number regulated transcriptional control, the high copy number histone gene plasmids should be transformed into hir2 or hir3 mutants. If the level of expression is repressed to the same extent as in HIR...
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