F hir2 and hir3 are two genes that were identied as

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Unformatted text preview: e if the other copy is regulated in the same way? e. How could you determine if there are sites in the DNA 5 ¿ to the H2A coding sequence that are responsible for the cell-cycle transcriptional regulation? f. HIR2 and HIR3 are two genes that were identified as regulators of transcription of the histone genes based on the properties of mutations in these genes. Null mutations in either gene lead to constitutive expression of H2A-H2B. Do HIR2 and HIR3 encode positive or negative regulators? g. Histone gene transcription is also sensitive to the number of copies of the histone genes in the cell. Introduction of a high copy number plasmid containing H2A and H2B into the cell will repress transcription of H2A and H2B. How could you determine if the copy number control of transcription is dependent on HIR2 and HIR3? Answer: This problem involves molecular analysis of gene structure and expression and the use of mutants to determine the role of a gene. a. Because histones have such an essential role in packaging DNA in the cell, you might predict...
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