Har06584refa001 020 6 11406 1009 am page 6 reference

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Unformatted text preview: iagram. har06584_refA_001-020 6 11/4/06 10:09 AM Page 6 Reference A Saccharomyces cerevisiae: Genetic Portrait of a Yeast saturation mutagenesis and to isolate rare mutants. Unlike E.coli, yeast can grow as either haploid or diploid cells, and both ploidy levels have useful applications in genetic analysis. A mutant hunt in yeast usually begins with mutagenesis of a diploid cell. For example, in a hunt for mutations in the uracil biosynthesis pathway, diploid yeast are mutagenized under conditions that are expected to yield single mutations. Meiosis (sporulation) is induced and the 4 meiotic products (spores) tested for Ura phenotype. If there is a recessive Ura mutation in the starting diploid cell, there would be 2 Ura , 2 Ura haploid spores (Fig. A.7a). A 2 : 2 pattern of phenotypes in the haploid meiotic products indicates there is a mutation in a single gene in the original diploid. If the Ura phenotype is seen in a diploid cell before meiosis, that cell contains a dominant mutation....
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This note was uploaded on 10/03/2013 for the course BI 206 taught by Professor Celenza during the Spring '08 term at BU.

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