BIO Laboratory Report 4-1 - LAZAR Soukaina Med Mehdi...

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BIO Laboratory Report (BIO 1401) Chromatography of Photosynthetic Pigments This experiment was conducted in order to identify the pigments available in a mint green leaf. The experiment contains two set of sub-experiments aiming at the separation of the pigments in the composition of the leaf, and the measurement of the absorption of the mixture of all the pigments in different wavelengths. LAZAR Soukaina Med Mehdi BENSEGHIR Instructor: Dr. Berrada, Fouad Spring 2012 Due date: 26 th April 2012
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BIO Laboratory Report (BIO 1401) 1 BIO Laboratory Report (BIO 1401) Chromatography of Photosynthetic Pigments Introduction: Green plants are living organisms that we call autotrophs or more precisely photoautotrophs. They are called autotrophs because they can produce organic molecules required for their life by themselves, and photo because they use the kinetic energy coming from the sun to undergo this process, which is called photosynthesis. As discussed in the lecture, photosynthesis requires the presence of certain pigments, from which the important is chlorophyll. It is not randomly that these kind of plants are green colored. This experiment will aim essentially at separating the pigments that were extracted from the leafs in a first sub-experiment and in the second, the measurement of the absorption of the leaf of the different wavelengths coming from the sunlight. Interesting experiments that will help us understand how pigments are absorbing wavelengths and discover which pigments we have, and their distances (R f ) in the chromatography paper. For this, we will use the chromatography technique, which is roughly speaking, the segregation of the components of a mixture in a chromatography paper using a solvent mixture all inside a jar. All this will be described throughout this report of our last reported lab experiment of this course (BIO 1401).
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BIO Laboratory Report (BIO 1401) 2 Methods: Needed equipment: - Mint leafs - Test tubes - Centrifuge - Chromatography paper - Solvents (Acetone, Petroleum ether) - Chromatography Jar - Spectrophotometer - Pipets
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