Lab 6 Prelab Questions.pdf - Lab 6 6 Calibration Methods...

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Lab 6: 6 Calibration Methods and Introduction to UV-Visible Spectroscopy Lauren Reynolds Prelab Questions 1.You will create 1.000L of 100.0 ppm stock solution of Acetylsalicylic acid (ASA, from the pure powder) in a 2 (v/v) % HCl in this lab. 2 (v/v) % HCl means the volume of concentrated HCl and the volume of the diluted HCl is 2:100. Determine the mass of the solute and volume of conc. HCl you are going to weigh out or measure with a graduated cylinder. Show your calculation. Mass of solute: 1L x 100.0mg L = 100.0mg or .1000g ASA Final concentration of 2% ( v v ) HCL: 2mL HCL 100mL x 10mL (to make 1000mL) = 20mL HCl b. Describe exactly how you will make the 1.000 L of 100.0 ppm ASA. When this is weighed out, you must get the mass you have found exactly! Weigh out .1000g of ASA and transfer to a volumetric flask. Methanol is added to increase solubility, via swirling the solution. Add a small amount of DI water and continue swirling. Finally, 20.00mL of HCl, measured from a graduated cylinder, is slowly added before diluting to the mark with more DI water. Invert three times. 2. Create dilution tables to create your standards for the external calibration

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