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Unformatted text preview: ls, how many more days (after 3,000 cells) until there will be 50,000 cells? (6 points) 42‐101 Final exam May 8, 2012 Name________________________________________________ Unless otherwise stated, there is only one correct answer per question 14. Rank the blood cells in order from largest number of circulating cells to least number of circulating cells (1‐ largest number) (3 points) ___ hematopoietic stem cells ___ leukocytes ___ tissue macrophages ___ red blood cells ___ platelets ‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐ mCherry is a fluorescent tag, like GFP. Chimera proteins can be produced by inserting a cDNA gene into the MCS. For the mCherry plasmid on the right answer the following six questions. 15. How big is the plasmid? (2 points) 16. Is this used for expression of proteins in mammalian cells or bacteria? (2 points) 17. List one way that you know if the plasmid is for mammalian or bacteria expression. (2 points) 18. Which antibiotic would allow replication of the plasmid DNA? (abbreviation is fine; 2 points) 19. Why would BamHI and AgeI not be useful restriction enzymes to add your gene of interest into the multicloning site? (3 points) 20. How would you separate this DNA from another 5500 bp segment of DNA based on size? (3 points) 42‐101 Final exam May 8, 2012 Name________________________________________________ Unless otherwise stated, there is only one correct answer per question 21. List the excitation and emission wavelengths of mCherry. (2 points) Excitation: Emission: 22. Compare the diffraction limits of mChe...
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This note was uploaded on 10/17/2013 for the course BME 42-101 taught by Professor Jalang during the Spring '10 term at Carnegie Mellon.
- Spring '10
- Biomedical Engineering