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enzyme has 2 active sites tether swing the biotin

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Unformatted text preview: zyme. - enzyme has 2 active sites: tether swing the biotin from site 1 to site 2 -required ATP to activate co2 pyruvate + co2 + ATP --> oxaloacetate +ADP *require biotin. 2 active sites and 1 tether biotin tether: swing around active sites, carry CO2 -isozyme: same enzyme but different structure and function. --> Mt or cytosolic malate dehydrogenase inside Mt: lots of CO2, high conc. of NADH from Kreb's cycle. -inside mitochondria has small pool size of NAD+, so Mt won't let NAD+ diffuse out into cytosol --> reduce oaa down to Malate, so it can diffuse out to cytosol -inner membrane: need transfer protein to help compounds to enter...
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