Protocol 1.1

Protocol 1.1 - Kurt Lowery Shane Hodges Ed Stanley BSCI412...

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Kurt Lowery Shane Hodges Ed Stanley BSCI412 0102 Group 9 Protocol 1.1 1.1.1 Determining the percent of killing for different UV exposure times Before beginning, put on protective eyewear. 1) Measure out 5.0 (significant figures are important) mL of the E. coli culture available in the laboratory and place into a centrifuge tube. Centrifuge for 10 minutes. 2) Decant and discard supernatant. 3) Place remaining pellet in 5.0 mL of saline solution. Place the contents of this test tube in a Petri dish and make sure it is covered. 4) Put together several tubes (3 to 5) for the measurement part of this experiment, and take a sample to measure the initial concentration of E. coli – label this tube 1. Cover the tube in foil to make sure the light does not degrade any material. 5) Put on gloves, turn off lights, expose the suspension to UV radiation for 30 seconds. Agitate the suspension during the exposure so the radiation spreads evenly. Take a sample of the suspension and label it tube 2. Cover this tube in foil as well.
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This note was uploaded on 04/08/2008 for the course BSCI 412 taught by Professor Lee during the Spring '08 term at Maryland.

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Protocol 1.1 - Kurt Lowery Shane Hodges Ed Stanley BSCI412...

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