blg 888.docx - 1 Results/Analyses Table 1 Fragment sizes...

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Results/AnalysesTable 1.Fragment sizes by size and position at DNA sequence of single and double digests.DigestFragment Sizes (by size)Fragment Sizes (position atDNA sequence)EcoRI21226, 7421, 5804, 5643, 4878,353021226, 4878, 5643, 7421,5804, 3530BamHI16841, 7233, 6770, 6527, 5626,55055505, 16841, 5626, 6527,7233, 6770HinDIII23130, 9416, 6682, 4361, 2322,2027, 56423130, 2027, 2322, 9416,564, 6682, 4361EcoRI + BamHI15721, 5505, 4669, 3775, 3758, 3530, 3240, 2752, 2564, 1868, 11205505, 15721, 1120, 3758,1868, 3775, 2752, 4669,2564, 3240, 3530EcoRI + HinDIII21226, 5148, 4973, 4268, 3530,2027, 1904, 1709, 1375, 947,831, 56421226, 1904, 2027, 947, 1375, 4268, 5148, 564, 1709, 4973, 831, 3530This data represents the fragment sizes by size and position at the DNA sequence, for the insilico digestion using the NEB cutter. The fragment sizes are for single digests of EcoRI, BamHI,HinDIII and double digests of EcoRI + BamHI and EcoRI + HinDIII. Table 2.Number of Fragments and restriction sites for single and double digests
DigestNumber of FragmentsNumber of Restriction SitesEcoRI65BamHI65HinDIII76EcoRI + BamHI1110EcoRI + HinDIII1211This data represents the number of fragments and restriction sites for single digests, EcoRI,BamHI, HinDIII and double digests, EcoRI + BamHI, EcoRI + HinDIII. Number of fragmentswere obtained using NEB cutter and number of restriction were calculated using the followingformula: # of restriction sites = # of fragments – 1. Table 3.Number of Fragments generated by single and double digests of 0.7% agarose gel.
LaneDigestionNumber of Fragments1BamHI52EcoRI + BamHI103EcoRI64EcoRI + HinDIII95HinDIII6Representing the number of fragments generated by the single digests (EcoRI, BamHI, HinDIII)and double digests (EcoRI + BamHI and EcoRI + HinDIII) for the restriction endonuclease oflinear lambda DNA on 0.7% agarose gel image in figure 2.
21226 4878 56437421 5804 35301____________________E________E__________E____________E_________E______48,5025505 15721 1120 3758 1868 3775 2752 4669 2564 3240 35301______B_______________ E___B_____E___B_____E____B______E___B____E___48,5025505 16841 5626 6527 7233 67701_______B____________________B________B_________B__________B__________48,502Fig. 1: Restriction Map for the Eco

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